ELISA

The enzyme-linked immunosorbent assay (ELISA) is a plate-based assay technique commonly used for measuring different types of analytes in various sample types. Interactions between the ligand immobilized in absorbant 96-well plates and the target in the sample is measured with a probe linked to a reporter enzyme. A substrate is added that changes color in the presence of the enzyme. Each ELISA normally detects a single analyte per well and does not distinguish between different analytes.

ELISA
Enzyme-linked immunosorbent assay (ELISA) in different setup

We offer to:

  • Develop new assays or kits for the ELISA platform, e.g. biomarker assays

  • Run existing ELISA kits for customers and collaborators - both in large- and small-scale setups 

ELISA procedure:

  • Ligands are immobilized in 96-wells plates
  • Samples (e.g. plasma, serum, milk, saliva) are added followed by detection reagents including a reporter enzyme and a substrate that changes color when modified by the enzyme (using intermittent wash steps)
  • Absorbance is read in an ELISA reader and results are reported as optical density values (OD values)
  • ELISA can be used with e.g. indirect, capture sandwich, competition and genomic assay setups